Antioxidant Activity and Estimation of Total Phenolic Content by Colorimetry
نویسنده
چکیده
The antioxidant potential of the 99% methanolic extract of leaves of Muntingia calabura was assessed by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and total phenolic content was measured by Folin-Ciocalteau (FC) by Singleton and Rossi using Gallic acid and Tannic acid as the calibration standard. Moreover Muntingia calabura leaf extract showed strong reducing power and significant antioxidant activity. In the DPPH radical scavenging assay, the IC50 value of the extract was found to be 22μg/ml. The total phenolic content was measured by Folin-Ciocalteau was found to be 0.903 for Gallic acid when compare to 2.900 for tannic acid.
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